Structural transitions and elasticity from torque measurements on dna

Elasticity structural torque

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For a full list of publications, please go to the laboratory website or Jeff’s google scholar profile. To conserve the stoichiometry, while permitting the DNA to change from an extended helix with ∼18. We have now extended our understanding of the torsional mechanics of DNA by measuring structural transitions and elasticity from torque measurements on dna rigidity as a function of tension, and are actively applying our rotor bead technique to study torque generation and changes in twist induced by DNA-binding molecules and DNA-associated enzymes. Hervé Beltz, Joel Azoulay, Serena Bernacchi, Jean-Pierre Clamme, Damien Ficheux, Bernard Roques, structural transitions and elasticity from torque measurements on dna Jean-Luc Darlix, Yves Mély. The torque–twist relationship for DNA shows two constant-torque regions, reflecting structural transitions at τ crit,+ = +34 pN nm and τ crit,-= -9. We analyze the dynamics of structural transitions between normal right-handed B form and unusual left-handed Z form for a linear DNA molecule. The dynamics under the external torque in physiological buffer is modeled by a Langevin equation, with the potential term given by the authors previously Phys. DNA (Lk0 ¼ Ntotal=10:4, where structural transitions and elasticity from torque measurements on dna structural transitions and elasticity from torque measurements on dna Ntotal structural transitions and elasticity from torque measurements on dna is the length of the full DNA construct in base pairs) and Lk ¼ for our experi-mental geometry.

View Record in Scopus Google Scholar. Structural transitions and elasticity from torque measurements on DNA, Zev Bryant et al, Nature 424,. Watson and Crick’s epochal presentation of the double helix structure in 1953 has paved dna the way to intense exploration of DNA’s vital functions in cells. Full-text available. , and structural transitions and elasticity from torque measurements on dna Bustamante, C. Mechanochemical analysis of DNA gyrase using rotor bead tracking, Jeff Gore et al, Nature 439,. The present solutions allow the. Effects of DNA-distorting proteins on DNA elastic response.

At approximately 65 pN, the B form (double-stranded DNA – dsDNA) has a transition to S-DNA. There have been dna a number of atomistic scale quantum. Cozzarelli, Carlos.

We thus measured the twist elasticity of DNA and characterized cooperative torque-induced structural transitions. Bryant Z, Stone MD, Gore J, Smith SB, Cozzarelli NR, and Bustamante C "Structural transitions and elasticity from torque measurements on DNA. Nature 424, 338–. “Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments”. E 84,. Using an X-ray interferometry technique, we measured the structural fluctuations of a short B-form duplex. In a plot oftorque as a func-.

Stone, Jeff structural transitions and elasticity from torque measurements on dna Gore, Steven B. Structural transitions and elasticity from torque structural transitions and elasticity from torque measurements on dna measurements on DNA, Zev Bryant, Michael D. Bryant Z, Stone MD, Gore J, Smith SB, Cozzarelli NR, Bustamante C.

Torque-twist signatures offree structural transitions and elasticity from torque measurements on dna boundary conditions and the behavior of a 100% AT sequence. Nature 424:338–341 CrossRef PubMedCentral PubMed Google Scholar. Nature 424, 338-341. We use foundational concepts from the Zimm-Bragg helix-coil transition theory and merge them with ideas from the structural transitions and elasticity from torque measurements on dna theory of fluctuating elastic rods to model structural transitions and elasticity from torque measurements on dna the mechanics of DNA. various enzymes on supercoiled DNA, albeit without a direct measurement of torque (for a review, see ref. 6 bp per 95Å pitch to a B-DNA–like helix with structural transitions and elasticity from torque measurements on dna structural transitions and elasticity from torque measurements on dna ∼10.

2 Rad51 monomers per 95Å pitch to a structure with ∼3. CAS PubMed PubMed Central Google structural transitions and elasticity from torque measurements on dna Scholar. dna One structural transitions and elasticity from torque measurements on dna family of exact solutions is obtained in terms of rational expressions for classical Jacobi elliptic functions. The mechanical deformation of DNA is very important in many biological processes. 5 bp per 35Å pitch, the filaments would need to change from a structure with ∼6. In this paper, we consider the reduced Kirchhoff equations of the noncircular cross-section elastic rod characterized by the inequality of the bending rigidities.

, Structural transitions and elasticity from torque measurements on DNA. BustamanteStructural transitions and elasticity from torque measurements on DNA Nature,, pp. Nature 424,. We also show that cooperative structural transitions in DNA can be exploited to construct.

We accomplish this in a direct measurement by integrating two nucleosomes into a DNA origami–based force spectrometer, which enabled subnanometer-resolution measurements of nucleosome-nucleosome distance frequencies via. pmid:View Article. Bryant Z, Stone MD, Gore J, Smith SB, Cozzarelli NR, Bustamante C () Structural transitions and elasticity from torque measurements on DNA. Chirality sensing by Escherichia coli topoisomerase IV and the mechanism of type II topoisomerases. 1038/nature01810 pmid:OpenUrl CrossRef PubMed Web of Science. Nature,, 338-341. Smith, Nicholas R.

5 Rad51 monomers per 35Å pitch. The results expose a potential nonlinearity of DNA elasticity and illustrate structural transitions and elasticity from torque measurements on dna how to measure the structural ensemble of a freely diffusing macromolecule. Marko Department of Physics and Astronomy and Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA Sebastien Neukirch´. 2 (color online). Sheet3 Sheet2 Sheet1 x y Figure 3(b) of Bryant, Z. Our objective is to examine the aforementioned structural transitions using ideas from statistical mechanics and the theory of elasticity.

Nature 424, 338 –. Measurements of force and extension on single molecules of structural transitions and elasticity from torque measurements on dna DNA have allowed direct determination of the molecule&39;s mechanical properties, provided rigorous tests of theories of polymer elasticity, revealed unforeseen structural transitions induced dna by mechanical stresses, and established an experimental and conceptual framework for mechanical assays of enzymes that act on DNA. Measurements of force and extension on single molecules of DNA have allowed direct determination of the molecule&39;s mechanical properties, provided rigorous tests dna of theories of polymer elasticity,.

Revealing the energy landscape for nucleosome association may contribute to the understanding of higher-order chromatin structures and their impact on genome regulation. To test our dynamic torque measurement method and establish baseline torque-twist relationships for a random DNA structural transitions and elasticity from torque measurements on dna sequence, we tested a structural transitions and elasticity from torque measurements on dna perfectly complementary double-stranded DNA segment, absent of obvious special sequence features. Viscous drag force and/or the angular Brownian motion of a bead have provided measurements of DNA torsional elasticity structural transitions and elasticity from torque measurements on dna as well as torque during DNA structural transitions2,3. To test our dynamic torque measurement method and establish baseline tor-que-twist relationships for a random DNA sequence, we tested a perfectly complementary double-stranded DNA segment, absent of obvious special sequence features. Here we report tests of the linearity of DNA’s twist elasticity, direct measurements of the torsional modulus (finding a value,40% higher than generally accepted), characterization of tor-que-induced structural transitions, and the establishment of a framework for future assays of torque and twist generation by DNA-dependent enzymes. 6 pN nm, and a nearly linear region.

1038/nature01810 pmid:OpenUrl CrossRef PubMed. In a plot of torque as a func-. (a) Average values for torque and other variables were calculated as a.

structural transitions and elasticity from torque measurements on dna We and others have developed angular optical trapping. Structural Transitions and Elasticity of Random DNA. Knowledge of the elastic properties of DNA is required to understand the structural dynamics of cellular processes such as replication and transcription. structural transitions and elasticity from torque measurements on dna Global force-torque phase diagram for the DNA double helix: Structural transitions, triple points, and collapsed plectonemes John F. To test our dynamic torque measurement method and establish baseline tor-que-twist relationships for a random DNA sequence, we tested a perfectly complementary double-stranded DNA segment, absent of dna obviousspecial sequence features. Structural transitions and elasticity from torque measurements on DNA. In 1953, Watson and Crick discovered the structure of the structural transitions and elasticity from torque measurements on dna DNA double helix that dna opened the area of molecular biology. "Structural transitions and elasticity from torque measurements on DNA.

Knowledge of the elastic properties of DNA is required to understand the structural dynamics of cellular processes such as replication and transcription. Measurements of force structural transitions and elasticity from torque measurements on dna structural transitions and elasticity from torque measurements on dna and extension on single molecules of DNA have allowed direct determination of the molecule&39;s mechanical properties, provided rigorous tests of theories of polymer elasticity, revealed unforeseen structural transitions induced by mechanical stresses, and established an experimental and conceptual framework for. Direct torque measurements 19 have been used to quantify the torsional rigidity of DNA 20-22, study the plectonemic structural transitions and elasticity from torque measurements on dna buckling transition 21-25, and analyze sequence-dependent structural transitions 23,26. , Structural transitions and elasticity from torque measurements on DNA. : Chen Z, Shaw A, Wilson H, Woringer M, Darzacq X, Marqusee S, Wang Q, Bustamante C. Also, recent advances of single molecule techniques have made it possible to probe structures and mechanics of constrained DNA at length scales ranging from nanometers to microns.

The S-DNA picture is consistent structural transitions and elasticity from torque measurements on dna with the observation of unpeeling during overstretching, unlike in the case of ssDNA.

Structural transitions and elasticity from torque measurements on dna

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Structural transitions and elasticity from torque measurements on dna - Transitions cuantos tipos


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